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. 2017 Dec 12;9(4):4354–4365. doi: 10.18632/oncotarget.23150

Figure 3. The polymorphism dampens the targeting efficiency and tumor suppressor function of the miR-29b-1/miR-29a cluster.

Figure 3

(A) MCL-1 and (B) CDK6 luciferase Assays. MiR-29b-1/miR-29a wild type (WT), polymorphism (Poly) or empty vector (EV) constructs were co-transfected with luciferase reporters containing the 3′untranslated regions (UTR) of MCL-1 or CDK6. The results are shown as relative luciferase values with respect to the EV after normalization with renilla. (C) Western Blotting for Mcl-1 and (D) Cdk6 proteins in K562 cells after transfection with miR-29b-1/miR-29a WT, Poly or EV constructs. GAPDH was used as loading control. As a control we also transfected cells with scramble (SC) or miR-29b (29b) oligonucleotides. (E) Apoptosis as measured using Annexin V/propidium iodine stain in K562 cells after transfection with miR-29b-1/miR-29a WT, Poly or EV constructs.