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. 2017 Dec 14;9(4):4411–4426. doi: 10.18632/oncotarget.23230

Figure 7. MALAT1 acts as ceRNA to enhance ATG7 expression by sponging miR142-3p.

Figure 7

(A) VSMCs were transfected with siMALAT1 for 24 h and subsequently the expression of LC3-I/LC3-II and p62 was detected using western blotting. (B) Twenty-four h after transfection with siMALAT1 or sicontrol, the cells were stimulated with PDGF-BB for another 24 h, and subsequently the expression of LC3-I/LC3-II, p62 and ATG7 was detected using western blotting. (C) According to the miRNA databases, miR142-3p is a potential miRNA target of MALAT1 and autophagy gene ATG7 is a potential target gene of miR142-3p. (D) The miR142-3p mimics were transfected into HVSMCs for 24 h, and subsequently the level of MALAT1 was detected using qRT-PCR. *P < 0.05. The miR142-3p mimics were transfected into VSMCs for 24 h, and subsequently the expression of ATG-7 detected using qRT-PCR (E) and western blotting (F). **P < 0.01. The miR142-3p mimics were transfected into VSMCs in the presence of PDGF-BB for 24 h. The level of MALAT1 was detected using qRT-PCR (G). *P < 0.05. The expression of ATG-7 was detected using qRT-PCR (H) and western blotting (I). *P < 0.05. The expression of OPN was detected using western blotting (J). *P < 0.05.