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. 2017 Dec 15;9(4):4461–4474. doi: 10.18632/oncotarget.23286

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Copyright: © 2018 Cai et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Specimens were longitudinally cut into 3 μm sections and the levels of Aggrecan expression level were detected by immunohistochemisty technique (original magnification ×200). As described in Material and Methods, the positive chondrocytes were counted and analyzed using Image-Pro Plus 6.0 Software and GraphPad Prism version 5. (A) Representative images from rats treated by PLCγ1 inhibitor for 1 month after ACLT+MMx (original magnification ×200). Graph indicating the percentage of positive chondrocytes expressing Akt. (B) Representative images from rats treated by PLCγ1 inhibitor for 2 month after ACLT+MMx (original magnification ×200). Graph indicating the percentage of positive chondrocytes expressing Akt.