Figure 2. Shh pathway is active in thyroid cancer cells and it is responsive to cyclopamine.

Thyroid cells were transfected with Gli-Luc reporter (pGL3-Gli-Luc) and 48 hours later Gli transcription factors activation was measured by Luciferase assay. Transfected cells were also treated with cyclopamine 10 mM for 24 h and stimulated with Shh ligand (pSec-Shh produced by HEK293T cells). Error bars represent standard deviations of experimental triplicates. ^**p = ≤ 0.01.