Figure 3. Thyroid cancer cells growth is sensitive to cyclopamine.

(A) IC50 (inhibitory concentration 50) of cyclopamine was assayed in thyroid cells treated with different doses of the drug (1–5–10–20 mM) for 72 hours. Cells were counted in triplicate. (B) For growth curve, 3 × 10⁴ thyroid cells were plated and treated with cyclopamine 10 mM. Cells were counted in triplicate every 24 hours. At 72 hours the number of cells was reduced compared to untreated cells. Three independent determinations were performed in triplicate.