Figure 4. O-GlcNAc regulates expression LXR-α, LXR-β, and SREBP-1 in HeLa cells.

Three experimental conditions (OGT knockdown (A)) and Thiamet G treatments (B), and hyperglycemia (C)) were used to examine regulation of LXR-α, LXR-β, and SREBP-1 expression. Western blot analysis was conducted using LXR-α, LXR-β, and SREBP-1 antibodies and β-actin was used as a loading control. For densitometry analysis, band intensity was normalized to the β-actin signal. Data are presented as mean ± S.E.M. (n = 3). ^*p < 0.05 and ^**p < 0.01.