Figure 5. OGT knockdown decreases O-GlcNAcylation of LXR-α and LXR-β.

Cellular extracts were subjected to immunoprecipitation with LXR-α (A) or LXR-β (B) antibodies, followed by immunoblot with anti-O-GlcNAc or OGT antibodies. (C) Cellular extracts were subjected to immunoprecipitation with anti-O-GlcNAc or -OGT antibodies, followed by western blot analysis with anti-LXR-α or -LXR-β antibodies. β-actin served as a loading control. (D) Levels of LXR-α, LXR-β and sCLU in OGT knockdown HeLa cells treated with Thiamet G. Data are presented as mean ± S.E.M. (n = 3). ^*p < 0.05 and ^**p < 0.01.