Figure 2. Depletion of pVHL leads to down-regulation of HR and MMR protein expression in renal carcinoma cells.

Western blotting was performed to analyze FANCD2, BRCA1, RAD51, and MLH1 expression in 786-O+VHL^WT cells 48 h post-transfection (A) or in RCC4+VHL^WT cells 72 h post-transfection (B) with GAPDH or VHL siRNA or mock treatment. Non-transfected 786-O^VHL–/– and RCC4^VHL–/– cells are included for comparison. Vinculin is presented as loading control and numbers below the blots indicate band density relative to vinculin and normalized to VHL^WT mock treatment samples.