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. 2017 Dec 19;9(4):4647–4660. doi: 10.18632/oncotarget.23470

Figure 3. DNA double-strand break repair is impaired in VHL-deficient renal carcinoma cells.

Figure 3

(A) The neutral comet assay was performed in 786-O and RCC4 matched pair cells 24 h post-treatment with 10 Gy IR (+IR) or mock treatment (Ctr). Median comet tail moments from analysis of ≥100 comets/sample are presented as box-and-whisker plots. Boxes, lower and upper quartiles; middle line, median; whiskers, 10th to 90th percentiles; **** significant at p < 0.0001; ns, not significant. (B) Representative images of comets observed in 786-O and RCC4 matched pair cells 24 h post-treatment with 10 Gy IR. (C) HR activity was measured in 786-O and RCC4 matched pair cells using the HR luciferase plasmid reactivation assay. Columns, mean of 3 replicates; bars, SEM.