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. 2017 Dec 21;9(4):4737–4757. doi: 10.18632/oncotarget.23531

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Copyright: © 2018 Bieling et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Evaluation of the immunogenic and cytotoxic activity of the peptide-induced EBV-specific T cells. The cytotoxic activity of peptide-induced EBV-specific T cells was investigated by means of IFN-γ and granzyme B FluoroSpot assays. PBMCs from healthy HLA-A*03:01-positive, EBV-seropositive donors (n = 5) were stimulated with one of the three peptide-mixtures (EBV_Consensus+3P_MIX, EBV_Consensus+4P_MIX, EBV_Consensus+11P_MIX, Table 1). The mere EBV_Consensus served as referential antigen. Spots identified with the filter for FITC represented IFN-γ producing cells and spots identified by the filter for Cy3 detected granzyme B producing cells. Results are given as the number of spots per well (spw), representing the number of spots in the antigen well after subtracting those of the respective negative control well. They are displayed as means ± SD.