Figure 5.

Identification of peptide-specific CTL precursor frequencies in healthy donors by pMHC multimer staining. Precursor frequencies of EBV-specific CTLs directed against the peptides A*03_BILF2_VTLA, A*03_BcRF1_FLLA and A*03_BALF3_QVAT were visualized via pMHC multimer staining, analyzed by flow cytometry and compared to the frequencies of the referential antigen A*03_EBNA3A_RLRA of freshly isolated PBMCs (day 0, n = 5). Findings are displayed as the mean percentage of CD8⁺multimer⁺ T cells ± SD after subtracting the respective percent value of the general nonsense dextramer, which functioned as a negative control.