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. 2017 Dec 19;9(4):4787–4797. doi: 10.18632/oncotarget.23465

Figure 2. NCTD induces cell cycle arrest and apoptosis in 786-O and A-498 cells.

Figure 2

(A) 786-O and A-498 cells were incubated with NCTD (0, 20, 40 and 80 μM) for 24 h, then cell cycle distribution was performed by flow cytometry. (B) Induction of cell apoptosis of 786-O and A-498 cells were measured with Annexin-V and PI double-stained flow cytometry after treated with NCTD. (C) 786-O and A-498 cells were treated NCTD for 24 h to detect the expressions of caspases and PARP were detected by western blot analysis. β-actin used as an internal control. (D) Pretreated with pan-caspase (Z-VAD-FMK) for 2 h, then treated with NCTD (40 μM) for another 22 h. Cell viability was measured by the MTT assay. (E) Apoptotic cells were detected by the Annexin-V and PI double-stained flow cytometry. All data are represented as mean ± SEM (n = 3) for each group.**p < 0.01 compared with control. #p < 0.01 compared with NCTD.