Figure 4. NCTD induce endoplasm reticulum stress in 786-O cells.

(A) 786-O cells were incubated with NCTD (0, 20, 40 and 80 μM) for 24 h. (B) The protein expression level of Grp78, ATF-4, CHOP, p-eIF2α and eIF2α were assessed by western blotting, β-actin used as an internal control. All data are represented as mean ± SEM (n = 3) for each group. ^**p < 0.01 compared with control.