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. 2017 Dec 19;9(4):4787–4797. doi: 10.18632/oncotarget.23465

Figure 5. NCTD induces endoplasm reticulum stress-mediated apoptosis pathway.

Figure 5

786-O cells were pretreated with salubrinal (2 μM) for 2 h before treated with NCTD (40 μM) for 24 h. (A) Cell viability was determined by MTT assay. (B) Apoptotic cells were detected by the Annexin-V and PI double-stained flow cytometry. (C) The level of ATF-4, CHOP, p-eIF2α and eIF2α expression were determined by western blot. β-actin used as an internal control. All data are represented as mean ± SEM (n = 3) for each group. **p < 0.01 compared with control. #p < 0.01 compared with NCTD.