Skip to main content
. 2017 Dec 13;9(4):4798–4813. doi: 10.18632/oncotarget.23219

Figure 6. miR-101-3p suppresses proliferation, migration and invasion of NSCLC cells.

Figure 6

(A) Cell proliferation analysis was performed with CCK-8 assay in A549 cells. Cells transfected with miR-101-3p or miR-NC were seeded into 96-well plate at 5000 cells/well and examined at time points of 0h, 24h, 48h and 72h. Overexpression of miR-101-3p significantly suppressed proliferation compared with that transfected with miR-NC. (B-C) Cell cycle assay was performed. Cells were transfected with miR-101-3p or miR-NC for 48 h, stained with PI and evaluated with a FACScalibur flow cytometer. Overexpression of miR-101-3p significantly induced the population of cells in the S/G1 compared with negative control. (D-G) Migrations assay were performed in H1299 and A549 cells respectively. Cells were transfected with miR-101-3p or miR-NC and seeded into the upper chamber of a transwell to count by transwell assay. Overexpression of miR-101-3p promotes cell migration compared with negative control. Data are presented as the mean ± SD (n = 3). Significance was defined as p<0.05 (*, p < 0.05; **, p < 0.01; ***, p < 0.001).