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. 2017 Dec 13;9(4):4798–4813. doi: 10.18632/oncotarget.23219

Figure 7. PTCH1 3’UTR mediated the effect of SLC39A6 on NSCLC migration.

Figure 7

(A-B) RT-PCR and western blot analysis of SLC39A6 expression level after knockdown of SLC39A6 by silencing RNA. (C) Cell proliferation analysis was performed with CCK-8 assay in A549 cells. Cells transfected with siSLC39A6 or negative control were seeded into 96-well plate at 5000 cells/well and examined at time points of 0h, 24h, 48h and 72h. Knockdown of SLC39A6 significantly suppressed proliferation compared with that transfected with negative control. (D-E) Cell cycle assay was performed in H1299 and A549 respectively. Cells were transfected with siSLC39A6 or siNC for 48 h, stained with PI and evaluated with a FACScalibur flow cytometer. Knockdown of SLC39A6 significantly induced an increase in G1 phase and a decrease in S phase compared with negative control. (F) Migrations of A549 cells after siSLC39A6 transfection were counted by transwell assay, knockdown of SLC39A6 significantly decreased cell migration compared with negative control. (G) Migrations of A549 cells after PTCH1-3’UTR-siSLC39A6 co-transfection were counted by transwell assay, in the presence of PTCH1-3’UTR, the effect of siSLC39A6 on migration could be partially rescued. Data are presented as the mean ± SD (n = 3). Significance was defined as p<0.05 (*, p < 0.05; **, p < 0.01; ***, p < 0.001).