Skip to main content
. 2017 Dec 18;9(4):4833–4850. doi: 10.18632/oncotarget.23373

Figure 3. Over-expression of SATB2 rescues TNF-α inhibition of osteoblastogenesis.

Figure 3

(A) SATB2 over-expression lentivirus and control lentivirus were transfected into C2C12 cells to construct cell lines that over-express SATB2 constantly, two cell lines were chosen and uncropped western blot images were shown. (B_E) Control cell lines and SATB2 over-expression cell lines from (A) were treated with or without 250 ng/ml BMP2 and TNF-α as indicated for 3 days, followed by MTT assay (B) and ALP staining (C). The ALP staining density in figure B were analyzed with image J software (D) and the SATB2 expression levels were detected using western blotting and uncropped western blot images were shown (E). (D) C2C12 cells were transfected with pCDNA3.1+ vector or pCDNA3.1+-SATB2 vector and then treated with TNF-α or BMP2 (250ng/ml) as indicated for 5 days, the OCN mRNA levels were measured with real time RT-PCR. The data are presented as mean ± S.D. (n=3, *p < 0.05; **p < 0.01; ***p < 0.001, T10 means 10 ng/ml TNF-α, BT10 means 250 ng/ml BMP2 and 10 ng/ml TNF-α).