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. 2017 Dec 26;9(4):5155–5168. doi: 10.18632/oncotarget.23703

Figure 5. Ectopic expression of miR-340 suppresses colon cancer cell proliferation and induces apoptosis in vitro.

Figure 5

(A, B) The short term effects of ectopic expression of miR-340 on theproliferation of HCT-116 and DLD-1 colon cancer cells examined by cell proliferation assay (MTS assay). Graph represents the percentage of viable cells in control and treated groups. (C) Effects of ectopic expression of miR-340 on the apoptosis of HCT-116 and DLD-1 colon cancer cells examined by TUNEL assay. The number of TUNEL positive cells were counted and made as graph. (D, E) Effects of overexpression of miR-340 on the colony formation or clonogenic ability of HCT-116 and DLD-1 colon cancer cells. Culture dishes represents the number of colonies formed upon transfection with pCMV and pCMV-miR-340. Graph represents the number of colonies formed in pCMV-miR-340 transfected cells normalized to the number of colonies formed by the pCMV transfected cells. The data are presented as means with SDs for three independent experiments. *p<0.05; **p<0.01; ***p<0.001. Scale bar 20μm.