Figure 4. Effect of SAM-treatment on promoter methylation and protein expression of cancer-related genes.

(A) Site-specific methylation analysis by pyrosequencing at the promoter of SPARC (location: chromosome 5: 151066730; corresponding to Illumina 450K ID: cg22116670). (B) Immunohistochemistry of control and SAM-treated tumors using antibodies against MUC1, FABP7 and SPARC proteins. (C) The stained areas were quantified using Fiji plugin (ImageJ). Results are shown as mean ± SEM (n=3). ^*P < 0.05.