Figure 2. Immunostaining of testicular VEGF in all groups.
(A) control group, (B) diabetes group, (C) diabetes + VEGF group. DAB was used as background staining. VEGF density (% of area) was determined using Image-Pro Plus to calculate the total area of positive cells. Data were presented as means ± SEM (D), three fields per section and five sections from each testis, n = 8 rats for each group.