Figure 6. Cell viability was measured by CCK8.
Rats sertoli cells (RTSCs) were divided into four groups. RTSCs in control groupVEGF group, Diabetes group and VEGF + Diabetes. Control group and VEGF group were treatment with normal medium. Diabetes group and VEGF + Diabetes group were cultured in high glucose medium with CoCl2. The cell viability of control group was viewed as the baseline (100%), and other groups compared with the control to show their cell viability in different culture environments. *P<0.05, +P<0.05 and #P<0.05 compared to control group; +P<0.05 compared to VEGF + CoCl2 group.