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. 2018 Feb 2;8:2274. doi: 10.1038/s41598-018-20715-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and IC₅₀ values in HUVECs and A549 cells treated with peptides. (A) Cell viability of HUVEC cells and A549 cells treated with HPRP-A1 alone or with iRGD or RGD was measured by MTT assay. Cells were cultured with 8 μM HPRP-A1 alone or in combination with iRGD (64 μM or 125 μM) or RGD (64 μM or 125 μM) for 1 h, 24 h or 48 h. (B) IC₅₀ values of peptide HPRP-A1 with or without iRGD at the indicated concentration were measured by MTT assay at various times. *P < 0.05; **P < 0.01; and ***P < 0.001.