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. 2018 Feb 2;8:2268. doi: 10.1038/s41598-018-20765-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The CXCR4 cell surface expression. (A) Flow cytometric histogram of PC3 versus PC3-S18-2-CL04 (the left panel). Notice the increase in CXCR4 expression in PC3-S18-2-CL04 cells, as there is shift in the histogram peak (red color). The median fluorescence intensities were analyzed and statistical analysis indicated that PC3-S18-2-CL04 cells (the right panel) express CXCR4 at a significantly higher level, than PC3. (B) As (A), but PC3-S18-2-CL03 cells were compared with PC3. The expression of CXCR4 was similar in PC3 and PC3-S18-2-CL03 (in blue color). All experiments were repeated 4 times. Graphs, presented in the right panels, were generated, using the statistical analysis with GraphPrism6 software.