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. 2018 Feb 2;8:2268. doi: 10.1038/s41598-018-20765-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CXCR4-mediated in vitro migration. The cells were cultured in trans-well plates and their ability to migrate towards chemotactic CXCL12 was assessed. (A) Cells were stained with crystal violet and the migration of PC3 (the left panel), PC3-S18-2-CL03 (the middle panel) and PC3-S18-2-CL04 (the right panel) was assessed based on the number of cells that crossed the membrane. Representative images show migrated cells that are indicated with arrows. (B) The intensity of crystal violet signal was quantified as a measure of the cells. We performed these experiments in triplicates and in two independent sets. The statistical analysis, using the GraphPrism 6 software showed the significant increase (p < 0.05) in motility of PC3-S18-2-CL04 cells, compared with PC3.