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. 2018 Feb 2;8:2246. doi: 10.1038/s41598-018-20424-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chronic administration of 0.5 g/kg alcohol decreases GFAP, while 1.5 g/kg alcohol increases astrogliosis. Immunostaining for GFAP (astrocyte marker, magenta) and DAPI (white) and quantifications thereof in control mice and in mice chronically treated with alcohol in cortex (A,B), corpus callosum (C,D) and hippocampus (E,F). CTR, saline; low, 0.5 g/kg ethanol; medium, 1.5 g/kg ethanol. *p < 0.05, **p < 0.01, one-way ANOVA with Tukey test. Scale bars, 100 µm. Bar graphs represent mean and standard error of the mean (SEM) of 3–4 mice per group.