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. 2018 Feb 2;8:2282. doi: 10.1038/s41598-018-20688-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simultaneous in situ hybridization of SPM samples in the water column of Hangzhou Bay. Fluorescence micrograph of (a) ammonia-oxidizing bacteria hybridization with Cy3-labeled probe NSO190 (red); (b) acetate-denitrifying cluster hybridization with FAM-labeled probe DEN124 (green); (c) methanol-denitrifying cluster hybridization with Cy5-labeled probe DEN67 (blue); (d) combined image of the three fluorescence micrographs, where the yellow cell aggregates are double labeled with NSO190 and DEN124, and the white cell aggregates are tripled labeled with NSO190, DEN124 and DEN67. A phase contrast-micrograph of the floc section, where the red bar = 20 μm, is depicted in (e).