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. 2018 Feb 2;8:2200. doi: 10.1038/s41598-018-20627-3

Figure 4.

Figure 4

Modulation of cytokine gene expression by JPtNPs in infected zebrafish.The various groups are: Control − uninfected zebrafish; Jac − zebrafish injected with jacalin alone; Bac − zebrafish infected with A. hydrophila alone; Bac + Jac − zebrafish infected with A. hydrophila and then treated with jacalin; Bac + JPtNPs − zebrafish infected with A. hydrophila and treated with JPtNPs. The concentrations of the various compounds are as mentioned in the methods section. The genes analyzed are (A) TLR-4a; (B) IL-1β, (C) TNF-α, (D) IFN-γ, (E) iNOS, and (F) IL-4. The colour of the bar represents the different time points at which gene expression was monitored: Black bar −3 h; gray bar −6 h and white bar −12 h. Bars indicate mean ±SD of five determinations where the samples were run in duplicates.