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. 2018 Feb 2;8:2239. doi: 10.1038/s41598-018-20722-5

Figure 4.

Figure 4

PD-PALM analysis of basal and LH-dependent cell surface LHR/FSHR heteromers. (a) Representative PD-PALM images and associated molecule heat maps from cells stably expressing FLAG-LHR and transiently expressing HA-FSHR. Cells were labelled live with CAGE500-conjugated FLAG and CAGE552-conjugated HA antibodies for 30 min at 37 °C prior to LH stimulation (100 nM at 0, 0.5 or 5 min). (b) Comparison of the percentage of total associated heteromeric molecules in cell lines stably expressing FLAG-LHR with FSHR via PD-PALM in the presence and absence of 100 nM LH treatment for 0, 0.5, or 5 min. (c) Analysis of the individual LHR/FSHR hetero-dimeric and hetero-oligomeric from (b). Each data point represents mean ± SEM from 8–12 cells, n = 3. *p < 0.05.