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. 2018 Feb 2;8:2277. doi: 10.1038/s41598-018-20768-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of NTS on adipogenic differentiation. (A) Schematic representation of the experimental design. (B–D) 3T3-L1 pre-adipocytes were treated with NTS for GM and DM and analyzed at the indicated time point after the start of adipocyte differentiation. (A) During four days of culture, adipocytes were stained with Oil Red O and then photographed for differentiation under a microscope. Scale bar = 100 μm. Inhibitory effect of NTS on (B) lipid content and (C) triglyceride deposition in differentiated 3T3-L1 cells. Results are presented as the mean ± SD of three independent experiments. Significant difference: ***p < 0.001 compared with the control group.