Figure 5.

Effect of the temperature on NTP hydrolysis activities of PatA/PatB reconstituted into nanodiscs. Hydrolysis activity of PatA/PatB (4 µg) incorporated into nanodiscs was measured at 340 nm upon addition of 4 mM ATP (grey bars) or 4 mM of GTP (black bars). Error bars represent the standard deviation of the mean for three independent measurements. (A) Nanodiscs were prepared from E. coli lipids and NTPase activities were determined at the indicated temperatures. (B) Nanodiscs were prepared from S. pneumoniae lipids and NTPase activities were determined at 30 °C and 37 °C with or without 4 mM orthovanadate.