Table 4.
Transmission test in chickens.
| dpi | O/BJ/293/13 | O/YN/438/14 | ||||||
|---|---|---|---|---|---|---|---|---|
| Direct inoculation | Physical contact | Direct inoculation | Physical contact | |||||
| Trachea | Cloaca | Trachea | Cloaca | Trachea | Cloaca | Trachea | Cloaca | |
| 1 | 3/3 | 0/3 | 0/3 | 0/3 | 2/3 | 0/3 | 0/3 | 0/3 |
| 3 | 3/3 | 3/3 | 3/3 | 2/3 | 3/3 | 2/3 | 0/3 | 0/3 |
| 5 | 3/3 | 1/3 | 2/3 | 1/3 | 3/3 | 1/3 | 0/3 | 0/3 |
| 7 | 3/3 | 0/3 | 2/3 | 0/3 | 2/3 | 0/3 | 2/3 | 0/3 |
| 9 | 0/3 | 0/3 | 2/3 | 0/3 | 1/3 | 0/3 | 1/3 | 0/3 |
| 11 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Five-week-old SPF chickens were divided into the direct inoculation group and physical contact group for each virus (O/BJ/293/13 and O/YN/438/14). The physical contact group was raised in the same cage as the chickens from the inoculation group, from 24 hours post-inoculation. The direct inoculation group was inoculated intranasally or intratracheally with 107.0 EID50/mL of virus. The chickens in direct inoculation group and the physical contact group were swabbed every other day in their tracheae and cloacae from 1 to 15 dpi using 1 mL of isolation media (50% glycerol in PBS, 0.5% gentamycin, and 1% mycostatin). The viruses recovered were titrated for infectivity in 10-day-old embryonated chicken eggs.