Table 1.
Proteins upregulated in rolB-expressing Arabidopsis calli.
| UniProtKB code | Name of the protein | Function or biological process | Activation, folds* | Notes1 | |
|---|---|---|---|---|---|
| 1 | Q9SN86 (MDHP_ARATH) | Malate dehydrogenase, chloroplastic/MDH | Carbohydrate metabolic process Tricarboxylic acid cycle |
14 ± 2 | Primary metabolism Response to cold |
| 2 | Q9LDV4 (ALAT2_ARATH) | Alanine aminotransferase 2, mitochondrial | Synthesizes pyruvate from L-alanine Photosynthesis |
12 ± 2 | Primary metabolism Response to hypoxia |
| 3 | Q9LVW7 (CARA_ARATH) | Carbamoyl-phosphate synthase small chain, chloroplastic | Amino-acid biosynthesis | 10 ± 0.5 | Primary metabolism Response to phosphate starvation |
| 4 | Q9LZ66 (SIR_ARATH) | Assimilatory sulfite reductase (ferredoxin), chloroplastic | Assimilatory sulfate reduction pathway during both primary and secondary metabolism | 10 ± 2 | Secondary metabolism Response to cold |
| 5 | Q9S7B5 (THRC1_ARATH) | Threonine synthase 1, chloroplastic | L-threonine biosynthesis | 1 ± 0.5 | Primary metabolism Stress-inducible1 |
| 6 | P24102 (PER22_ARATH) | Peroxidase 22 | Hydrogen peroxide catabolic process | 5.6 ± 0.7 | Plant defense |
| 7 | Q9SJZ2 (PER17_ARATH) | Peroxidase 17 | Hydrogen peroxide catabolic process | 3.4 ± 0.6 | Plant defense |
| 8 | Q9C6Z3 (ODPB2_ARATH) | Pyruvate dehydrogenase E1 component subunit beta-2, chloroplastic | Fatty acid biosynthetic process Glycolysis |
5.3 ± 0.7 | Primary metabolism |
| 9 | Q9LFG2 (DAPF_ARATH) | Diaminopimelate epimerase, chloroplastic | Amino-acid biosynthesis | 4 ± 1.5 | Primary metabolism Stress-inducible1 |
| 10 | Q9SRY5 (GSTF7_ARATH) | Glutathione S-transferase F7 | Defense response to bacterium Defense response to fungus, incompatible interaction Response to salt stress |
3.5 ± 0.4 | Plant defense |
| 11 | P42760 (GSTF6_ARATH) | Glutathione S-transferase F6 | Defense response to bacterium Response to oxidative stress Response to salt stress Response to water deprivation |
3.5 ± 0.6 | Plant defense Involved in camalexin biosynthesis |
| 12 | Q9FUS6 (GSTUD_ARATH) | Glutathione S-transferase U13 | Detoxification Stress response |
3 ± 0.2 | Plant defense |
| 13 | Q9FWR4 (DHAR1_ARATH) | Glutathione S-transferase DHAR1, mitochondrial | Scavenging of ROS under oxidative stresses | 2 ± 0.3 | Plant defense Key component of the ascorbate recycling system |
| 14 | Q9STW6 (HSP7F_ARATH) | Heat shock 70 kDa protein 6, chloroplastic/Hsp70-6 Synonym: cpHsc70-1 |
Host-virus interaction, protein transport, stress response | 2.4 ± 0.15 | Chaperone |
| 15 | Q9LTX9 (HSP7G_ARATH) | Heat shock 70 kDa protein 7, chloroplastic/Hsp70-7 | Host-virus interaction, protein transport, stress response | 2.9 ± 0.6 | Chaperone |
| 16 | Q9SIF2 (HS905_ARATH) | Heat shock protein 90-5, chloroplastic Hsp90-5/CR88 Synonym: Hsp88.1 |
Response to heat Response to salt stress Response to water deprivation Embryo development |
2.4 ± 0.7 | Chaperone |
| 17 | P21238 (CPNA1_ARATH) | Chaperonin 60 subunit alpha 1, chloroplastic, Cpn60 | Chloroplast organization Embryo development |
2.3 ± 0.6 | Chaperone |
| 18 | O65282 (CH20_ARATH) | 20 kDa chaperonin, chloroplastic, Cpn10 | Stress response Required to activate the iron superoxide dismutases (FeSOD) |
2 ± 0.6 | Chaperone Functions along with Cpn60 |
| 19 | O04130 (SERA2_ARATH) | D-3-phosphoglycerate dehydrogenase 2, chloroplastic | Amino-acid biosynthesis | 2.5 ± 1 | Primary metabolism |
| 20 | Q9M9K1 (PMG2_ARATH) | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 | Glycolysis | 2.1 ± 0.5 | Primary metabolism |
| 21 | Q42592 (APXS_ARATH) | L-ascorbate peroxidase S, chloroplastic/mitochondrial | Plays a key role in hydrogen peroxide removal | 2 ± 0.5 | Plant defense |
| 22 | Q9XI87 (Q9XI87_ARATH) | VH1-interacting kinase (VIK) |
Auxin-activated signaling pathway, negative regulation of programmed cell death, plant-type hypersensitive response, response to cold and water deprivation | 2 ± 0.6 | Signal transduction, MAPK cascade |
| 23 | Q9ZUN8 (Q9ZUN8_ARATH) | HEAT STRESS TOLERANT, DWD1 Synonym: HTD1/WD-40 repeat family protein |
Cul4-RING E3 ubiquitin ligase complexHeat stress response | 2 ± 0.4 | Signal transduction |
| 24 | Q9S7D8 (APS4_ARATH) | ATP sulfurylase 4, chloroplastic/APS4 | Hydrogen sulfide biosynthetic process Regulation of hypersensitive response |
2.2 ± 1.0 | Positive regulation of flavonoid biosynthesis |
| 25 | Q9SA34 (IMDH2_ARATH) | Inosine-5′-monophosphate dehydrogenase 2 | Purine biosynthesis | 2.1 ± 1.0 | Primary metabolism |
| 26 | P93819 (MDHC1_ARATH) | Malate dehydrogenase, cytoplasmic 1 | Tricarboxylic acid cycle | 2 ± 0.6 | Primary metabolism Stress-inducible1 |
| 27 | P57106 (MDHC2_ARATH) | Malate dehydrogenase, cytoplasmic 2 | Tricarboxylic acid cycle | 2 ± 0.7 | Primary metabolism Stress-inducible1 |
| 28 | Q94JQ3 (GLYP3_ARATH) | Serine hydroxymethyltransferase 3, chloroplastic | Glycine metabolic process | 2 ± 0.2 | Primary metabolism |
| 29 | O22832 (STAD7_ARATH) | Acyl-[acyl-carrier-protein] desaturase 7, chloroplastic, FAB2 | Fatty acid biosynthetic process | 2 ± 0.3 | Plant defense |
| 30 | P41088 (CFI1_ARATH) | Chalcone-flavonone isomerase 1/TRANSPARENT TESTA 5 | Flavonoid biosynthesis | 2 ± 0.15 | Secondary metabolism |
| 31 | Q8VY84 (KCY1_ARATH) | Probable UMP-CMP kinase 1 | Pyrimidine nucleotide biosynthetic process | 1.8 ± 0.12 | Primary metabolism |
1Data from UniProt and TAIR.
2Less and Galili59.
*Mean ± standard deviation of three biological repeats.