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. 2018 Feb 2;8:2265. doi: 10.1038/s41598-018-20527-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Emergence of large DNA fragments containing (6-4)PPs and CPDs at later time points with decreased generation of excision repair products. UV-irradiated HeLa cells were harvested at the indicated time points and subjected to extraction of soluble DNA fragments released from genomic DNA. The extracted DNA molecules were then immunoprecipitated with anti-(6-4)PPs (A) or anti-CPDs antibodies (C) and analyzed as described in Fig. 1(A). The relative intensities of the excised oligonucleotides and the large DNA molecules containing (6-4)PPs and CPDs were quantified and shown in (B) and (D), respectively.