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. 2018 Feb 2;8:2327. doi: 10.1038/s41598-018-20653-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Carbon isotopic discrimination (Δ¹³C) and (b) total leaf N content along sugarcane leaf blade. B0, Base “zero”; B, Base; M, Middle; T, Tip. 10 N and 270 N correspond to treatments of 10 and 270 mg of N per kg of sand, respectively. DW, dry weight. Data are presented as the mean ± SE. Letters indicate statistical significance using ANOVA followed by Fisher’s exact test (n = 3; P ≤ 0.05).