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. 2018 Feb 2;8:2242. doi: 10.1038/s41598-018-20563-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the complete KNDy population in vehicle- and estradiol-treated ovariectomized rats. (A) Diagram of the dorsal to ventral (d/v) division of the arcuate nucleus (ARC) (coronal plane) by 200 µm intervals. (F) The percentage of KNDy cells located in the d/v intervals of the ARC in OVX + VEH and OVX + E2 rats. (G) The number of KNDy neurons in the rostral-caudal and ventral-dorsal regions of the ARC in OVX + VEH and OVX + E2 rats. The total number of Kp + NKB (KNDy) cells in the mARN and cARN is significantly decreased in OVX + E2 rats compared to OVX + VEH rats. n = 4/group. *p < 0.05.