Table 1.

Tetrahydrofuran-mediated dehydration of rat and sheep tissue for optical tissue clearing.

Tissue	Size	% Tetrahydrofuran in ddH20
Mouse brain (Erturk et al. 2012)	1.4 cm × 1 cm × 0.8 cm	50%, 70%, 80%, 100% 1 h, 100% o/n, 100% 1 h
Rat brain block	1.9 cm × 0.9 cm × 0.6 cm	50% o/n, 60%, 70%, 80%, 90%, 96% 1 h, 100% o/n
Whole rat brain	2.5 cm × 1.5 cm × 1.2 cm	50% o/n, 60%, 70%, 80% 2 h, 80% o/n 90%, 96% 100% 2 h, 100% o/n
Sheep hypothalamic block	1.5 cm × 1.5 cm × 1 cm	50% o/n, 60%, 70%, 80%, 2 h, 80% o/n, 90%, 96%, 100%, 100%, 100%, 100% 2 h