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. 2018 Feb 2;8:2310. doi: 10.1038/s41598-018-20840-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid composition of the reticulon mutants rtn19 and rtn20 and rescue lines. (A) Sterols and the major phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from roots and leaves of two week-old seedlings (wild type, mutant lines rtn20 and rtn19, and rescue line) were quantified. Data was obtained from 3 independent experiments by HPTLC coupled to densitometry. Sterol, PC and PE amounts were determined as µg/g FW, the values for the control roots and leaves were normalized to 100 and the corresponding values for the mutant and rescue lines were calculated accordingly. (B) The ratios between sterols to PC + PE content are indicated for the different lines.