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. 2018 Feb 2;8:2310. doi: 10.1038/s41598-018-20840-0

Figure 9.

Figure 9

Lipid composition of 3betahsd/d1 mutant lines. (A) Sterols and the major phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from roots and leaves of two week-old seedlings were quantified. The lines used are wild type Col0, two lines heterozygous for 3betahsd/d1 (HetZ-1 and HetZ-2) and two lines homozygous for 3betahsd/d1 (HZ-1 and HZ-2). Data were obtained from 3 independent experiments by HPTLC coupled to densitometry. Sterol, PC and PE amounts were determined as µg/g FW, the values for the control roots and leaves were normalized to 100 and the corresponding values for the mutant and rescue lines were calculated accordingly. (B) The ratios between sterols to PC + PE content are indicated for the different lines. Using GC-MS different sterol species (cholesterol, 4α-methylzymosterol, campesterol, sigmasterol, gamma-sitosterol, beta-sitosterol, fucosterol) were further quantified in roots (C) and leaves (D) of two week-old seedlings of the wild type control Col0 and heterozygous (HetZ-1 and HetZ-2) and homozygous (HZ-1 and HZ-2) lines for 3betahsd/d1. Data are presented as percentage of the overall composition.