Figure 3.

(A) Images showing phosphorylated NF-κB immunofluorescence in eosinophils in the bronchoalveolar lavage (BAL); (B) phosphorylated NF-κB in eosinophils evaluated by immunofluorescence; in asthmatic (OVA) and asthmatic mice treated with oral administration of Ang-(1–7)/hydroxypropyl β-cyclodextrin (HPβCD) [60 µg/kg of Ang-(1–7) and 92 µg/kg of HPβCD]. Propidium iodide (PI), a marker of nucleus cell, in red; siglec F, a marker of eosinophil, in blue; and p-p65 in green. MFI = mean fluorescence intensity. Scale = 15 µm. Bars show mean ± SEM from five to six animals per group. ^#p ≤ 0.05 compared to asthmatic untreated mice (OVA; Student’s t-test).