Figure 6.

Surface plasmon resonance analyses of the interaction between 92R or 91R and hCCR9 synthetic peptides. A synthetic peptide corresponding to hCCR9A aa 2–22 was immobilized on a carboxymethyl-dextrane CM5 chip by an amino coupling method. Afterward, different concentrations of 91R (A) or 92R (B) mAb were added, ranging from 0.41 to 33 nM. The black sensograms show the fitted curves obtained with the Biaevaluation 4 software. Competitive experiments were analyzed by binding of 91R (C) or 92R (D) to a biotinylated synthetic peptide hCCR9(2–19)-captured onto the surface of a SA sensor chip. As competitors, 1 µM of peptides hCCR9(2–22), hCCR9(10–30) and hCCR9(13–30) were used. The differences between experimental and control flow cells is given in resonance units (RU).