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. 2018 Feb 2;15:32. doi: 10.1186/s12974-018-1077-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Time course expression of pIRE1α in brain tissues and Immunofluorescence staining showing co-localization post HI. a Western blot assay for temporal profile of pIRE1α and IRE1α expressions from ipsilateral hemisphere at 0, 3, 6, 12, 24, and 72 h post HI; data are expressed as mean ± SEM, n = 5 per group, per time point. Relative densities have been normalized against the sham group. *P < 0.05 compared with sham group. b–d Representative microphotographs of immunofluorescence staining for pIRE1α (red) co-localization on neurons (NeuN, green; b), microglia (Iba-1, green; c), and astrocytes (GFAP, green; d) at 6 h post HI. n = 3 per group, scale bar = 100 μm