Table 2.
Immunohistochemical procedures
| Ag | Ab clone | Ab supplier | Ag retrieval | Ab dilution incubation time | Reveal system |
|---|---|---|---|---|---|
| ER | SP1 | Ventana | CC2 36 min | ready-to-use, 32 min | ultraView DAB |
| PR | 1E2 | Ventana | CC2 36 min | ready-to-use, 20 min | ultraView DAB |
| HER2 | 4B5 | Ventana | CC2 36 min | ready-to-use, 20 min | ultraView DAB |
| Ki67 | SP6 | Thermo Scientific | CC2 36 min | 1/100, 60 min | ultraView DAB |
| CD8 | SP16 | Thermo Scientific | CC2 36 min | 1/400, 60 min | ultraView DAB |
| CD4 | SP35 | Cell Marque | CC2 36 min | 1/50, 60 min | ultraView DAB |
| FoxP3 | 2A11G9 | Novus Biologicals | CC2 36 min | 1/25, 60 min | ultraView Red |
| CD38 | SP149 | Cell Marque | CC2 36 min | 1/50, 60 min | ultraView DAB |
| CD20 | L26 | Dako | CC2 36 min | 1/100, 32 min | ultraView DAB |
Abbreviations: Ag antigen, Ab antibody, ER oestrogen receptor, PR progesterone receptor, CC1 or CC2 Cell Conditioning Buffer 1 or 2 (Ventana). All ultraView systems are from Ventana