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Alcohol increases Pde4b expression and decreases cAMP levels in the brain. A, mRNA levels of brain Pde4b quantified by real time qPCR and B, Immunoblot analysis of brain homogenates from WT mice that were PF and AF for 1 week. (n = 5–7 in each group). C, brain cAMP levels measured by using cAMP ELISA kit were normalized by protein content. Data are presented as means ± SD (n = 5–7 in each group). *P < 0.05.
