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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neuropharmacology. 2017 Aug 12;125:376–385. doi: 10.1016/j.neuropharm.2017.08.011

Figure 5.

Figure 5

Increase of Pde4b expression in primary microglial cells in response to LPS, Tnfα and Hmgb1. mRNA levels of Pde4a, b and d were quantified by real time qPCR in A, cells treated with LPS, 100 ng/ml for 90 minutes. B, cells treated with recombinant Tnfα, 20 ng/ml for 90 minutes. C, cells treated with recombinant Hmgb1, 10 μg/ml for 90 minutes. Data are presented as mean ± SD from 3 independent experiments, *P<0.05, and *** P<0.001 compared UT. Representative images of IHC for Pde4b protein in D, cells treated with LPS, 100 ng/ml for 3 hours, E, in cells treated with Tnfα, 20 ng/ml for 3 hours, F, in cells treated with Hmgb1, 10 μg/ml for 3 hours.