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. 2018 Jan 26;9:28. doi: 10.3389/fmicb.2018.00028

FIGURE 6.

FIGURE 6

Effect of the NO scavenger 2-phenyl-4, 4, 5, 5,-tetramethylimidazoline-1-oxyl 3-oxide (PTIO) on nitrite production. Different concentrations of PTIO were added after 63 h in early to mid-exponential growth phase indicated by the arrow. Cultures with 20 μM PTIO resumed nitrite production 72 h after the addition of PTIO, while higher concentrations resulted in a complete arrest. Nitrite curves of control and 20 μM PTIO cultures show mean values from duplicates, whereas 100 and 400 μM curves are from triplicates and error bars represent the standard error of the mean.