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. 2018 Jan 25;9:26. doi: 10.3389/fpls.2018.00026

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Copyright © 2018 Ji, Zhao, Gao, Cao, Mao, Zhou, Fan, Borkovich, Ouyang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Responses of slmiR482 family members to FOL infection. (A) Alignment of slmiR482 family members. Sequences were aligned to maximize identical residues. (B) Northern blot analysis of slmiR482 family members. Root total RNA samples (20 μg) from Moneymaker treated with water (MM-water), Moneymaker infected with FOL (MM-FOL), Motelle treated with water (Mot-water) and Motelle infected with FOL (Mot-FOL) were used to prepare Northern blots. Oligonucleotide probes were used to quantitate levels of individual slmiR482 family members. Blots were probed with U6 to serve as a loading control. (C) Quantification of relative miRNA amounts. miRNA levels obtained from Northern analysis in (B) were normalized using water treatment as 100% (1.0).