Table 1.
Primers used in this study.
| Primer Name | Application | Sequence (5′ – 3′) |
|---|---|---|
| slmiR482a | Northern Blot Probe | TAGGAATGGGTGGAATTGGAAA |
| slmiR482b | ″ | GGCATGGGCGGTGTAGGCAAGA |
| slmiR482c | ″ | GGCATGGGCGGTGTAGGCAAGA |
| slmiR482d-5p | ″ | TTTTTCCATCCCACCCACTCC |
| slmiR482d-3p | ″ | TTGGCATGGGTGGAATAGGAAA |
| slmiR482e-5p | ″ | AATCTTTCCACCCCACCCACA |
| slmiR482e-3p | ″ | GGTATGGGAGGAGTAGGAAAGA |
| U6 | ″ | GGGGCCATGCTAATCTTCTCTG |
| Solyc12g099060-F | CAAAACAACAGTTGCCCAGC | |
| Solyc12g099060-R | ″ | GAAAGAATGCCTTGAGTACGC |
| Sl18S-rRNA-F | ″ | TGACGGAGAATTAGGGTTCG |
| Sl18S-rRNA-R | ″ | CCTCCAATGGATCCTCGTTA |
| Solyc12g099060-F | q-PCR Primer | CAAAACAACAGTTGCCCAGC |
| Solyc12g099060-R | ″ | GAAAGAATGCCTTGAGTACGC |
| Mi-1-F | ″ | GAAACAACTGTCATTGCAT |
| Mi-1-R | ″ | GAAACAACTGTCATTGCAT |
| Mot-I2-F | ″ | CCTCCTTTTCTCACCTCACT |
| Mot-I2-R | ″ | CAATCGATATTTATGATGGG |
| Nt-Actin-F | ″ | GAAAACTGGACAGAACTG |
| Nt-Actin-R | ″ | CATCCTTGAGGCTCATTCG |
| IGS1049 | ″ | TGCGATTTGGACGAGATATGTG |
| IGS1050 | ″ | ATTTGCCTACCCTGTACCTACC |
| Solyc12g099060-attB1 | VIGS Vector Construction | GGGGACAAGTTTGTACAAAAAAGCAGGCTGCAATCTCGTGAATCAGAGAATGTG |
| Solyc12g099060-attB2 | ″ | GGGGACCACTTTGTACAAGAAAGCTGGGTCACATTCTCTGATTCACGAGATTGC |
| slmiR166a-F | Co-expression Vector Construction | TTAAGAATTCGTTGAAGTCAAGCTAAGATGCG |
| slmiR166a-R | ″ | TTAAACTAGTCGAAACAAGTTTTAGTAGGTGCC |
| slmiR482e-3p-F | ″ | TTAAGAATTCCGGTTAGATCCAAGTTCTGG |
| slmiR482e-3p-R | ″ | TTAAACTAGTATAATGTAACCCCACCGACC |
| Solyc12g099060-F | ″ | TTAAGAATTCATGGGCGTGAAGGAGGAGCT |
| Solyc12g099060-R | ″ | TTAAACTAGTACGTCCTGCCACATTCAGCT |