Figure 2.

Upregulation and downregulation of ALDH1A2 levels in epithelial ovarian cancer cell lines using lentiviral particles.

Notes: All experiments were repeated three times. The data are presented as mean ± standard deviation. (A) Relative ALDH1A2 mRNA expression using shRNA in A2780 cell lines; (B) downregulation of ALDH1A2 protein expression by shRNA in A2780 cell lines; (C) relative ALDH1A2 protein expression using shRNA in A2780 cell lines; (D) relative SKOV3 mRNA expression using shRNA in A2780 cell lines; (E) relative ALDH1A2 mRNA expression using shRNA in SKOV3 cell lines; (F) downregulation of ALDH1A2 protein expression by shRNA in SKOV3 cell lines; (G) relative ALDH1A2 protein expression using shRNA in SKOV3 cell lines; (H) ALDH1A2 protein expression in overexpressed and downregulated epithelial ovarian cancer cell lines; *P<0.05, significantly different compared with control group oe represented as ALDH1A2 overexpression group; oe-nc represented as overexpression control group; sh represented as ALDH1A2 knockdown group; and sh-nc represented as knockdown control group.

Abbreviations: ALDH1A2, aldehyde dehydrogenase 1 family member A2; shRNA, short hairpin RNA; ctrl, control.