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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Aug 4;141(4):1427–1438. doi: 10.1016/j.jaci.2017.06.042

Figure 5. Effect of p.V85D on Sec61 complex-dependent co-translational protein translocation into the ER.

Figure 5

A: Representative Western blot and respective quantification of SEC61A1 levels in HeLa cells treated with the indicated siRNA and transiently transfected with the indicated plasmids. B: Representative phosphorimaging blot and respective quantification of the efficiency of co-translational transport of pre-prolactin (ppl) into the ER by HeLa cells given as relative percentage of mature, processed protein prolactin (pl). C: Representative phosphorimaging blot (n=3) of the efficiency of Sec61 complex-independent post-translational transport of pre-Cytochrome B5 (pre) into the ER (in HeLa cells) given as the relative percentage of mature, glycosylated protein (glyc). Data are represented as mean ± SD; ns: not significant, *p<0.05, **p<0.005 (unpaired t test).