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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Aug 4;141(4):1427–1438. doi: 10.1016/j.jaci.2017.06.042

Figure 7. Effect of UPR induction on plasma cells and sensitized Hek293T cells after SEC61A1-V85D overproduction.

Figure 7

A: Flow cytometric analysis of the indicated cell lines after stable transduction with an expression vector encoding SEC61A1-wt or -V85D and GFP separated by an IRES site. B: Representative Western blots and respective statistical analysis showing levels of the indicated proteins in U266 cells stably overexpressing SEC61A1-wt and -V85D normalized to tubulin levels and relative to cells transduced with the empty vector. C: Relative mRNA expression of the indicated transcription factors in L363 cells stably overexpressing SEC61A1-wt or -V85D. D: Representative Western blots showing levels of the indicated proteins in Thapsigargin-treated Hek293T cells stably overexpressing SEC61A1-wt and/or -V85D (n=4). The bar graph shows the respective statistical analysis of XBP1s levels normalized to tubulin levels and relative to cells transduced with the empty vector. E: Western blot (n=1) showing levels of indicated proteins in JK6L cells treated for 24 hours with siRNA against SEC61A1. Data in bar graphs is represented as mean ± SD, ns: not significant, *p<0.05, **p<0.005, ***p<0.001 (unpaired t test).